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Western blot analysis of extracts of various cell lines, using SLC5A1 antibody (18-954) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunohistochemistry of paraffin-embedded rat kidney using SLC5A1 antibody (18-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SLC5A1 antibody (18-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SLC5A1 antibody (18-954) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat kidney using SLC5A1 Polyclonal Antibody (18-954) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse kidney using SLC5A1 Polyclonal Antibody (18-954) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SLC5A1 antibody (18-954) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

SLC5A1 Antibody

CATALOG NUMBER: 18-954

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human SLC5A1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
U-937
Positive Control 3:
Mouse kidney
Positive Control 4:
Mouse brain
Predicted Molecular Weight:
Observed: 66kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC5A1
Additional Names:
SLC5A1, D22S675, HSGLT1, Na(+)/glucose cotransporter 1, Na+/glucose cotransporter 1, SGLT1, NAGT, Sodium-glucose transporter 1, Sodium/glucose cotransporter 1
Ncbi Gene Id Number:
6523
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-954

List Size:
50 uL

List Price:

$285.00

Out of stock

Datasheet
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