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Western Blot at 1:2000 dilution + Y79 whole cell lysate Lysates/proteins at 20 ug per lane.
SLC5A12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution + Y79 whole cell lysate Lysates/proteins at 20 ug per lane.

SLC5A12 Antibody

CATALOG NUMBER: 55-133

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SLC5A12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human SLC5A12.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC5A12
Additional Names:
Sodium-coupled monocarboxylate transporter 2, Electroneutral sodium monocarboxylate cotransporter, Low-affinity sodium-lactate cotransporter, Solute carrier family 5 member 12, SLC5A12, SMCT2
Protein Accession Number:
Q1EHB4
Protein Gi Number:
189046187
Ncbi Gene Id Number:
159963
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Normal blood lactate is maintained at about 1.5 mM, andlittle filtered lactate is excreted in urine. Reabsorption oflactate is mediated by the low-affinity Na(+)-coupled lactatetransporter SLC5A12 in the initial part of the proximal tubule andby the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed17692818]).
Background Reference 1:
Gopal, E., et al. Biochim. Biophys. Acta 1768(11):2690-2697(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-133

List Size:
400 ul

List Price:

$495.00

Datasheet
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