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Western blot analysis of extracts of various cell lines, using SLC6A2 antibody (15-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using SLC6A2 antibody (15-429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

SLC6A2 Antibody

CATALOG NUMBER: 15-429

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SLC6A2 (NP_001165975.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
A-549
Positive Control 2:
SH-SY5Y
Positive Control 3:
Mouse kidney
Positive Control 4:
Mouse spinal cord
Predicted Molecular Weight:
Observed: 69kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC6A2
Additional Names:
SLC6A2, NAT1, NET, NET1, SLC6A5
Ncbi Gene Id Number:
6530
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-429

List Size:
100 uL

List Price:

$436.00

Datasheet
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