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42-964 (1ug/ml) staining of Human Substantia nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-964 (1ug/ml) staining of Human Substantia nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SLC6A3 Antibody

CATALOG NUMBER: 42-964

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QLTSSTLTNPRQSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 85kDa band observed in Human Brain (Substantia nigra) lysates (calculated MW of 68.5kDa according to NP_001035.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28):7040-6. PMID:18614672).. Recommended concentration: 1-3ug/ml.
Positive Control 1:
Human Substantia Nigra Lysate
Predicted Molecular Weight:
Approx 85 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC6A3
Additional Names:
SLC6A3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3, DAT, DAT1, DA transporter, sodium-dependent dopamine transporter, solute carrier family 6 member 3
Protein Accession Number:
NP_001035.1
Protein Gi Number:
4507041
Ncbi Gene Id Number:
6531

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-964

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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