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46-377 (2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-377 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue
46-377 (2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SLC6A4 Antibody

CATALOG NUMBER: 46-377

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-LHQGERETWGK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in Human Colon lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC6A4
Additional Names:
SLC6A4, SERT, solute carrier family 6 (neurotransmitter transporter, serotonin), member 4, HGNC:11050, 5-HTT, HTT, OCD1, hSERT, 5-hydroxytryptamine transporter, 5HT transporter, Na+/Cl- dependent serotonin transporter, serotonin transporter, sodium-dependent serotonin transporter, solute carrier family 6 member 4, 5-HTTLPR, OTTHUMP00000163633
Protein Accession Number:
NP_001036.1
Protein Gi Number:
4507043
Ncbi Gene Id Number:
6532
Background Reference 1:
Voeller KK. Attention-deficit hyperactivity disorder (ADHD). J Child Neurol. 2004 Oct;19(10):798-814. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-377

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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