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Western blot analysis of extracts of various cell lines, using SLC7A2 antibody (15-611) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using SLC7A2 antibody (15-611) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using SLC7A2 antibody (15-611) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using SLC7A2 antibody (15-611) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using SLC7A2 antibody (15-611) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SLC7A2 antibody (15-611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

SLC7A2 Antibody

CATALOG NUMBER: 15-611

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC7A2 (NP_001008539.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse brain
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 72kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC7A2
Additional Names:
SLC7A2, ATRC2, CAT2, HCAT2, CAT-2
Ncbi Gene Id Number:
6542
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a cationic amino acid transporter and a member of the APC (amino acid-polyamine-organocation) family of transporters. The encoded membrane protein is responsible for the cellular uptake of arginine, lysine and ornithine. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-611

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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