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42-149 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
42-149 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

SLC7A5 Antibody

CATALOG NUMBER: 42-149

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-RHRKPELERPIKVN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Spleen lysates (calculated MW of 55.0kDa according to NP_003477.4). Recommended concentration: 1-3ug/ml.Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC7A5
Additional Names:
SLC7A5, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5, 4F2LC, CD98, D16S469E, E16, LAT1, MPE16, hLAT1, 4F2 light chain, CD98 light chain, L-type amino acid transporter 1, integral membrane protein E16, large neutral amino acids transporter 1, large neutral amino acids transporter small subunit 1, sodium-independent neutral amino acid transporter LAT1
Protein Accession Number:
NP_003477.4
Protein Gi Number:
71979932
Ncbi Gene Id Number:
8140
Background Reference 1:
Ohno Y, Suda K, Masuko K, Yagi H, Hashimoto Y, Masuko T. Production and characterization of highly tumor-specific rat monoclonal antibodies recognizing the extracellular domain of human L-type amino-acid transporter 1. Cancer Sci. 2008 May;99(5):1000-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-149

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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