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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
SLC8A3 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC8A3 Antibdy for immunohistochemistry.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

SLC8A3 Antibody

CATALOG NUMBER: 56-660

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SLC8A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585-614 amino acids from the C-terminal region of human SLC8A3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
103 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC8A3
Additional Names:
Sodium/calcium exchanger 3, Na(+)/Ca(2+)-exchange protein 3, Solute carrier family 8 member 3, SLC8A3, NCX3
Protein Accession Number:
P57103
Protein Gi Number:
34395973
Ncbi Gene Id Number:
6547
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the sodium/calcium exchangerintegral membrane protein family. Three mammalian isoforms infamily 8 have been identified. Na+/Ca2+ exchange proteins areinvolved in maintaining Ca2+ homeostasis in a wide variety of celltypes. The protein is regulated by intracellular calcium ions andis found in both the plasma membrane and intracellular organellarmembranes, where exchange of Na+ for Ca2+ occurs in an electrogenicmanner. Alternative splicing has been observed for this gene andmultiple variants have been described.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Pulina, M.V., et al. J. Biol. Chem. 281(28):19645-19654(2006)
Background Reference 3:
Gomez-Villafuertes, R., et al. J. Neurosci. 25(47):10822-10830(2005)
Background Reference 4:
Lindgren, R.M., et al. Gene 348, 143-155 (2005) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-660

List Size:
400 ul

List Price:

$495.00

Datasheet
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