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42-218 (1ug/ml) staining of HEK293 (A) and A431 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-218 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue
42-218 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black li
42-218 (1ug/ml) staining of HEK293 (A) and A431 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SLCO1B3 Antibody

CATALOG NUMBER: 42-218

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-NGTSPEIVEKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in lysates of cell line HEK293 and A431 (calculated MW of 74.2kDa according to NP_001336849.1 and 77.4kDa acording to NP_062818.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of A431 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLCO1B3
Additional Names:
SLCO1B3, solute carrier organic anion transporter family, member 1B3, LST-3TM13, LST3, OATP1B3, OATP8, SLC21A8, liver-specific organic anion transporter 3TM13, organic anion transporter LST-3c, solute carrier family 21 (organic anion transporter), member 8
Protein Accession Number:
NP_062818.1,NP_001336849.1
Protein Gi Number:
9790233
Ncbi Gene Id Number:
28234
Background Reference 1:
Aoki M, Terada T, Ogasawara K, Katsura T, Hatano E, Ikai I, Inui K, Impact of regulatory polymorphisms in organic anion transporter genes in the human liver. Pharmacogenetics and genomics 2009 Aug 19 (8): 647-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-218

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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