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46-380 (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-380 (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SLIMMER Antibody

CATALOG NUMBER: 46-380

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: CTVSRVSHPVSKARK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Heart lysates is consistent with literature (PMID:10480922; calculated MW of 36.3kDa according to AAC72886). Recommended concentration: 0.01-0.1ug/ml.
Specificity:
This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FHL1
Additional Names:
FHL1, KYO-T, SLIM1, bA535K18.1, four and a half LIM domains 1, Four-and-a-half LIM domains 1, bA535K18.1 (LIM protein SLIMMER), RP11-535K18.1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, SLIMMER, XMPMA, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein1
Protein Accession Number:
AAC72886.1
Protein Gi Number:
3859849
Ncbi Gene Id Number:
2273
Background Reference 1:
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1.Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in thecytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem. 1999 Sep 17;274(38):27083-91.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-380

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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