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Western blot analysis in mouse heart tissue lysates (35ug/lane).
SLIT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse heart tissue lysates (35ug/lane).

SLIT1 Antibody

CATALOG NUMBER: 57-020

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This SLIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-113 amino acids from the N-terminal region of human SLIT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
168 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLIT1
Additional Names:
Slit homolog 1 protein, Slit-1, Multiple epidermal growth factor-like domains protein 4, Multiple EGF-like domains protein 4, SLIT1, KIAA0813, MEGF4, SLIL1
Protein Accession Number:
O75093
Ncbi Gene Id Number:
6585
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
Background Reference 1:
Marlow, R., et al. Cancer Res. 68(19):7819-7827(2008)
Background Reference 2:
Hussain, S.A., et al. J. Biol. Chem. 281(51):39693-39698(2006)
Background Reference 3:
Lin, L., et al. Stem Cells 24(11):2504-2513(2006)
Background Reference 4:
Lim, J., et al. Cell 125(4):801-814(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-020

List Size:
400 ul

List Price:

$495.00

Datasheet
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