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Western blot analysis of Slitrk5 in 3T3 cell lysate with Slitrk5 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
Immunohistochemistry of Slitrk4 in human brain tissue with Slitrk4 antibody at 2.5 μg/mL.
Immunofluorescence of Slitrk5 in Human Brain cells with Slitrk5 antibody at 5 μg/mL.
Western blot analysis of Slitrk5 in 3T3 cell lysate with Slitrk5 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
Trial Size

Slitrk5 Antibody

CATALOG NUMBER: 4475

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Slitrk5 antibody was raised against a 14 amino acid synthetic peptide from near the center of human Slitrk5.
The immunogen is located within amino acids 310 - 360 of Slitrk5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Slitrk5 antibody can be used for detection of Slitrk5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to have no cross-reactivity to other Slitrk proteins.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
Slitrk5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Slitrk5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Slitrk5 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
Ncbi Official Symbol:
SLITRK5
Additional Names:
Slitrk5 Antibody: LRRC11, bA364G4.2, KIAA0918, LRRC11, SLIT and NTRK-like protein 5, Leucine-rich repeat-containing protein 11
Protein Accession Number:
O94991
Protein Gi Number:
46397810
Ncbi Gene Id Number:
26050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Slitrk5 Antibody: SLIT and NTRK-like family 5 (Slitrk5) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the protein family. Like every other Slitrk protein except Slitrk1, overexpression of Slitrk5 inhibited neurite outgrowth in cultured neurons, suggesting that these proteins are involved in the control of neurite outgrowth.
Background Reference 1:
Aruga J and Mikoshiba K. Identification and characterization of Slitrk, a novel transmembrane protein family controlling neurite outgrowth. Mol. Cell Neurosci.2003; 24:117-29.
Background Reference 2:
Aruga J, Yokota N, and Mikoshiba K. Human SLITRK family genes: genomic organization and expression profiling in normal and brain tumor tissue. Gene2003; 315:87-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4475

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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