Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of Slug in 293 cell lysate with Slug antibody at in (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunofluorescence of Slug in human kidney tissue with Slug antibody at 20 μg/mL.
Western blot analysis of Slug in 293 cell lysate with Slug antibody at in (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

Slug Antibody

CATALOG NUMBER: 3959

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (92%)
Immunogen:
Slug antibody was raised against a 13 amino acid synthetic peptide from near the center of human Slug.
The immunogen is located within amino acids 90 - 140 of Slug.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Slug antibody can be used for detection of Slug by Western blot at 0.5 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This Slug antibody is predicted to not cross-react with Snail.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 3:
Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 4:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 29 kDa
Observed: 30 kDa
Purification:
Slug Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Slug Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Slug antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNAI2
Additional Names:
Slug Antibody: SLUG, WS2D, SLUGH1, SNAIL2, SLUG, SLUGH, Zinc finger protein SNAI2, Neural crest transcription factor Slug
Protein Accession Number:
O43623
Protein Gi Number:
11134406
Ncbi Gene Id Number:
6591
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.
Background Reference 1:
Neito MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 1994; 264:835-9.
Background Reference 2:
Martinez-Estrada OM, Culleres A, Soriano FX, et al. The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells. Biochem. J. 2006; 394:449-57.
Background Reference 3:
Shioiri M, Shida T, Koda K, et al. Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients. Br. J. Cancer 2006; 94:1816-22.
Background Reference 4:
Wu WS, Heinrichs S, Xu D, et al. Slug antagonizes p53-mediated apoptosis of hematopoietic progenitors by repressing puma. Cell 2005; 123:641-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3959

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369