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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad1 (Phospho-Ser465).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad1 (Phospho-Ser465).

Smad1 (phospho Ser465) Antibody

CATALOG NUMBER: 79-498

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Smad1 (Phospho-Ser465) antibody was raised against a peptide sequence around phosphorylation site of serine 465 (I-S-S-V-S (p) ) derived from Human Smad1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Smad1 only when phosphorylated at serine 465.
Predicted Molecular Weight:
55 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SMAD1
Additional Names:
BSP1, JV41, BSP-1, JV4-1, MADH1, MADR1, ERBB, ERBB1, HER1, BSP1, Dwarfin-A, Dwf-A
Protein Accession Number:
NP_001003688.1
Protein Gi Number:
51173727
Ncbi Gene Id Number:
4086
Background:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
Background Reference 1:
Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113 (5): 718–726Linyu Li, et al. (2004) Mol Cell Biol. January; 24 (2): 856–864. Claire Nourry, et al. (2004) BMC Cell Biol. 5: 20

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-498

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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