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Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) Antibody. The lane on the right is treated with the antigen-specific peptide.

Smad2 (phospho Ser250) Antibody

CATALOG NUMBER: 79-976

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Smad2 (phospho Ser250) antibody was raised against a peptide sequence around phosphorylation site of serine250 (E-L-S (p)-P-T) derived from Human Smad2 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.
Predicted Molecular Weight:
65 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SMAD2
Additional Names:
JV18-1, MADH2, Mad-related protein 2, Mothers against DPP homolog 2,
Protein Accession Number:
NP_001003652.1
Protein Gi Number:
51173730
Ncbi Gene Id Number:
4087
Background:
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Background Reference 1:
Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagué J (1999)Genes Dev 13, 804-16 .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-976

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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