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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Thr220).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Thr220).

Smad2 (phospho Thr220) Antibody

CATALOG NUMBER: 79-500

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Smad2 (Phospho-Thr220) antibody was raised against a peptide sequence around phosphorylation site of threonine 220 (P-E-T (p) -P-P) derived from Human Smad2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SMAD2
Additional Names:
JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, ERBB, ERBB1, HER1, Mad-related protein 2, Mothers against DPP homolog 2
Protein Accession Number:
NP_001003652.1
Protein Gi Number:
51173730
Ncbi Gene Id Number:
4087
Background:
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Background Reference 1:
Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15 (2): 420–434. Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24 (3): 1106–1121. Kimberly A Brown et al. (2004) Breast Cancer Res. ; 6 (3): R215–R231.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-500

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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