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Western blot analysis in CEM cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of SMAD3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
SMAD3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

SMAD3 Antibody

CATALOG NUMBER: 57-106

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Pig, Rat
Immunogen:
This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human SMAD3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SMAD3
Additional Names:
Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3
Protein Accession Number:
P84022
Protein Gi Number:
51338669
Ncbi Gene Id Number:
4088
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the SMAD, afamily of proteins similar to the gene products of the Drosophilagene 'mothers against decapentaplegic' (Mad) and the C. elegansgene Sma. SMAD proteins are signal transducers and transcriptionalmodulators that mediate multiple signaling pathways. This proteinfunctions as a transcriptional modulator activated by transforminggrowth factor-beta and is thought to play a role in the regulationof carcinogenesis.
Background Reference 1:
Ge, Q., et al. J. Cell. Physiol. 225(3):846-854(2010)
Background Reference 2:
Lee, J., et al. J. Biol. Chem. 285(34):26618-26627(2010)
Background Reference 3:
Roder, C., et al. Childs Nerv Syst (2010) In press :
Background Reference 4:
Valdes, A.M., et al. Arthritis Rheum. 62(8):2347-2352(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-106

List Size:
400 ul

List Price:

$495.00

Datasheet
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