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Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-Smad3 (Thr179) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-Smad3 (Thr179) Antibody. The lane on the right is treated with the antigen-specific peptide.

Smad3 (phospho Thr179) Antibody

CATALOG NUMBER: 80-005

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Smad3 (phospho Thr179) antibody was raised against a peptide sequence around phosphorylation site of threonine179 (P-E-T (p)-P-P) derived from Human Smad3 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Smad3 only when phosphorylated at threonine 179.
Predicted Molecular Weight:
50 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SMAD3
Additional Names:
JV15-2, MAD-3, MADH3, Smad 3, Mothers against decapentaplegic homolog 3
Protein Accession Number:
NP_001138574.1
Protein Gi Number:
223029440
Ncbi Gene Id Number:
4088
Background:
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Background Reference 1:
Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24
Background Reference 2:
Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol
Background Reference 3:
Cancer Res 8, 1375-87
Background Reference 4:
Matsuura I, et al. (2010) J Biol Chem 285, 1754-64

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-005

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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