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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human U2OS at 1:1000.
Antibody used in WB on Human U2OS.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

SMAD4 Antibody

CATALOG NUMBER: 27-474

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
60 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SMAD4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SMAD4
Additional Names:
SMAD4, JIP, DPC4, MADH4, MYHRS
Protein Accession Number:
NP_005350
Protein Gi Number:
4885457
Ncbi Gene Id Number:
4089
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
Background Reference 1:
Kantola, A.K., et al., Gene 363, 142-150 (2005).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-474

List Size:
100 ul

List Price:

$473.00

Datasheet
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