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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on r-SMAD5 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of transfected 293T lysate (SMAD8) , Lane 9: 5ug of transfected 293T lysate (GFP)).
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

SMAD5 Antibody

CATALOG NUMBER: 27-475

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SMAD5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SMAD5
Additional Names:
SMAD5, DWFC, JV5-1, MADH5
Protein Accession Number:
NP_005894
Protein Gi Number:
47778925
Ncbi Gene Id Number:
4090
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
Background Reference 1:
Zimonjic, D.B., et al., (2003) Neoplasia 5 (5), 390-396.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-475

List Size:
100 ul

List Price:

$519.00

Datasheet
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