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Antibody used in WB on Human Lung at 1 ug/ml.
Antibody used in WB on Human Lung at 1 ug/ml.

SMAD7 Antibody

CATALOG NUMBER: 27-478

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:62500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
46 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SMAD7 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SMAD7
Additional Names:
SMAD7, FLJ16482, MADH7, MADH8, CRCS3
Protein Accession Number:
NP_005895
Protein Gi Number:
5174517
Ncbi Gene Id Number:
4092
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.
Background Reference 1:
Suh, Y.A., (2006) Biochem. Biophys. Res. Commun. 341 (3), 889-894.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-478

List Size:
100 ul

List Price:

$473.00

Datasheet
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