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46-385 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
46-385 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

SMARCA3 Antibody

CATALOG NUMBER: 46-385

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KQAKINEIRTLIDL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1ug/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).
Specificity:
NP_003062.2 and NP_620636.2 are varients that represent the same protein.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HLTF
Additional Names:
HLTF, SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SNF2-like 3, sucrose nonfermenting-like 3, helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor-1 regulator, SNF2L3
Protein Accession Number:
NP_003062.2,NP_620636.1
Protein Gi Number:
21071052
Ncbi Gene Id Number:
6596
Background Reference 1:
Sheridan PL, Schorpp M, Voz ML, Jones KA. Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter. J Biol Chem. 1995 Mar 3;270(9):4575-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-385

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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