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Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.
Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.<br><br>Green: SMARCA4 Antibody (7749) <br> Blue: DAPI staining
Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.
Trial Size

SMARCA4 Antibody

CATALOG NUMBER: 7749

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.
The immunogen is located within amino acids 1420 - 1470 of SMARCA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 185 kDa
Observed: 200 kDa
Purification:
SMARCA4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SMARCA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SMARCA4
Additional Names:
SMARCA4 Antibody: BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1, SNF2B, Transcription activator BRG1, ATP-dependent helicase SMARCA4
Protein Accession Number:
NP_001122321
Protein Gi Number:
192807323
Ncbi Gene Id Number:
6597
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).
Background Reference 1:
Muchardt C and Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. EMBO J. 1993; 12:4279-90.
Background Reference 2:
Wilson BG and Roberts CW. SWI/SNF nucleosome remodellers and cancer. Nat. Rev. Cancer 2011; 11:481-92.
Background Reference 3:
Bultman S, Gebuhr T, Yee D, et al. A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes. Mol. Cell 2000; 6:1287-95.
Background Reference 4:
Bochar DA, Wang L, Beniya H, et al. BRCA1 is associated with a human SWI/SNF-related complex: linking chromatin remodeling to breast cancer. Cell 2000; 102:257-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7749

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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