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Western blot analysis of lysate from SK-BR-3 cell line, using SMARCE1 Antibody at 1:1000 at each lane.
SMARCE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of lysate from SK-BR-3 cell line, using SMARCE1 Antibody at 1:1000 at each lane.

SMARCE1 Antibody

CATALOG NUMBER: 57-670

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SMARCE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-335 amino acids from the C-terminal region of human SMARCE1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SMARCE1
Additional Names:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BAF57, SMARCE1, BAF57
Protein Accession Number:
Q969G3
Protein Gi Number:
61247587
Ncbi Gene Id Number:
6605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is part of the largeATP-dependent chromatin remodeling complex SWI/SNF, which isrequired for transcriptional activation of genes normally repressedby chromatin. The encoded protein, either alone or when in theSWI/SNF complex, can bind to 4-way junction DNA, which is thoughtto mimic the topology of DNA as it enters or exits the nucleosome.The protein contains a DNA-binding HMG domain, but disruption ofthis domain does not abolish the DNA-binding ornucleosome-displacement activities of the SWI/SNF complex. Unlikemost of the SWI/SNF complex proteins, this protein has no yeastcounterpart.
Background Reference 1:
Hah, N., et al. Cancer Res. 70(11):4402-4411(2010)
Background Reference 2:
Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)
Background Reference 3:
Pan, H., et al. Biochim. Biophys. Acta 1772(9):1075-1084(2007)
Background Reference 4:
Camargo, L.M., et al. Mol. Psychiatry 12(1):74-86(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-670

List Size:
400 ul

List Price:

$495.00

Datasheet
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