Smooth Muscle Actin Antibody [SMAT2-1]

CATALOG NUMBER: 33-983

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Rat
Immunogen:
Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P
Application Note:
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ACTA2
Additional Names:
ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin, ACTVS, GIG46
Ncbi Gene Id Number:
59
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-983

List Size:
100 ug

List Price:

$626.00

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