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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

SMPD2 Antibody

CATALOG NUMBER: 30-066

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMPD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SMPD2 antibody can be used for detection of SMPD2 by ELISA at 1:1562500. SMPD2 antibody can be used for detection of SMPD2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
48 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SMPD2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SMPD2
Additional Names:
SMPD2, NSMASE, ISC1, NSMASE1
Protein Accession Number:
NP_003071
Protein Gi Number:
92859618
Ncbi Gene Id Number:
6610
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMPD2 converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Background Reference 1:
Kimura, K., (2006) Genome Res. 16 (1), 55-65.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-066

List Size:
100 ul

List Price:

$473.00

Datasheet
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