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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

SMPD3 Antibody

CATALOG NUMBER: 60-306

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SMPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the N-terminal region of human SMPD3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SMPD3
Additional Names:
Sphingomyelin phosphodiesterase 3, Neutral sphingomyelinase 2, nSMase-2, nSMase2, Neutral sphingomyelinase II, SMPD3
Protein Accession Number:
Q9NY59
Protein Gi Number:
73921262
Ncbi Gene Id Number:
55512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Filosto, S., et al. J. Biol. Chem. 285(14):10213-10222(2010)
Background Reference 3:
Maupas-Schwalm, F., et al. Cell. Signal. 21(12):1925-1934(2009)
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-306

List Size:
400 ul

List Price:

$495.00

Datasheet
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