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Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.
Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.
Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.
Trial Size

SMURF1 Antibody

CATALOG NUMBER: 7945

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.
The immunogen is located within amino acids 180 - 230 of SMURF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 80, 83 kDa
Observed: 78 kDa
Purification:
SMURF1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SMURF1
Additional Names:
SMAD specific E3 ubiquitin protein ligase 1, SMAD ubiquitination regulatory factor 1, E3 ubiquitin-protein ligase SMURF1
Protein Accession Number:
NP_065162
Protein Gi Number:
31317292
Ncbi Gene Id Number:
57154
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).
Background Reference 1:
Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.
Background Reference 2:
Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.
Background Reference 3:
Yamashita M, Ying SX, Zhang G, et al. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell 2005; 121:101-113.
Background Reference 4:
Kwon A, Lee HL, Woo KM, et al. SMURF1 plays a role in EGF-induced breast cancer cell migration and invasion. Mol. Cells 2013; 36:548-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7945

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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