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Western blot analysis of lysates from MDA-MB-231 cells, using SMURF1 Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 30s.
Western blot analysis of lysates from MDA-MB-231 cells, using SMURF1 Mouse mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 30s.

SMURF1 Mouse Recombinant Antibody (82-412)

CATALOG NUMBER: 82-412

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
MDA-MB-231
Predicted Molecular Weight:
86kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG2a Kappa
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.1% sodium azide and 0.1% gelatin.
Concentration:
Batch dependent
Storage Conditions:
Store at 4℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SMURF1
Additional Names:
SMURF1; E3 ubiquitin-protein ligase SMURF1
Protein Accession Number:
Q9HCE7
Ncbi Gene Id Number:
57154
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-412

List Size:
100 uL

List Price:

$436.00

Datasheet
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