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Western blot analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 μg/ml.
Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 μg/mL.
Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/mL.
Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 μg/ml.<br><br>Green: SMURF2 Antibody (7773) <br> Blue: DAPI staining
Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/ml.<br><br>Green: SMURF2 Antibody (7773) <br> Blue: DAPI staining
Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 μg/ml.
Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 μg/ml.
Western blot analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 μg/ml.
Trial Size

SMURF2 Antibody

CATALOG NUMBER: 7773

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
The immunogen is located within amino acids 140 - 190 of SMURF2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
Positive Control 1:
Cat. No. 1285 - C2C12 Cell Lysate
Predicted Molecular Weight:
Predicted: 82 kDa
Observed: 85 kDa
Purification:
SMURF2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SMURF2
Additional Names:
SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2
Protein Accession Number:
NP_073576
Protein Gi Number:
12232397
Ncbi Gene Id Number:
64750
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).
Background Reference 1:
Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.
Background Reference 2:
Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.
Background Reference 3:
Lin X, Liang M, and Feng XH. Smurf2 is a ubiquitin E3 ligase mediating proteasomedependent degradation of Smad2 in transforming growth factor-beta signaling. J. Biol. Chem. 2000; 275:36818-22.
Background Reference 4:
Kavsak P, Rasmussen RK, Causing CG, et al. Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation. Mol. Cell 2000; 6:1365-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7773

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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