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Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody at 1:1000.
Western blot analysis of SMYD3 antibody in CEM cell line lysates (35ug/lane)
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody at 1:1000.

SMYD3 Antibody

CATALOG NUMBER: 62-842

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SMYD3
Additional Names:
Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1
Protein Accession Number:
Q9H7B4
Protein Gi Number:
212276523
Ncbi Gene Id Number:
64754
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
Background Reference 1:
Zou,J.N., Wang,S.Z. Cancer Lett. 280 (1), 78-85 (2009)
Background Reference 2:
Wang,S.Z., Luo,X.G. BMB Rep 41 (4), 294-299 (2008)
Background Reference 3:
Wang,H., Liu,Y. Cancer Sci. 99 (4), 787-791 (2008)
Background Reference 4:
Barlesi,F., Giaccone,G. Int. J. Cancer 122 (6), 1441-1442 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-842

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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