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Western blot analysis of extracts of various cell lines, using SMYD4 antibody (18-610) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody (18-610). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody (18-610). Western blot was performed from the immunoprecipitate using SMYD4 antibody (18-610) at a dilition of 1:500.____Detection: ECL Enhanced Kit._Exposure time: 20s.
Western blot analysis of extracts of various cell lines, using SMYD4 antibody (18-610) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

SMYD4 Antibody

CATALOG NUMBER: 18-610

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
MCF7
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Predicted Molecular Weight:
Observed: 90kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SMYD4
Additional Names:
SET and MYND domain-containing protein 4, 211-, SMYD4, KIAA1936
Ncbi Gene Id Number:
114826
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-610

List Size:
100 uL

List Price:

$436.00

Datasheet
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