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46-392 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-392 (0.1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-392 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-392 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SNAI1 Antibody

CATALOG NUMBER: 46-392

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: RKPSDPNRKPNY-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in lysates of cell line A431 and in Rat Kidney lysates (calculated MW of 29.1kDa according to Human NP_005976.2 and Rat NP_446257.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Rat: Cui F et al (2015). PMID: 26417374.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat: Cui F et al (2015). PMID: 26417374.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SNAI1
Additional Names:
SNAI1, snail homolog 1 (Drosophila), SLUGH2, SNA, SNAH, dJ710H13.1 , snail 1 (drosophila homolog), zinc finger protein, snail 1 homolog, snail 1 zinc finger protein
Protein Accession Number:
NP_005976.2
Protein Gi Number:
18765741
Ncbi Gene Id Number:
6615
Background Reference 1:
Vega S, Morales AV, Ocana OH, Valdes F, Fabregat I, Nieto MA. Snail blocks the cell cycle and confers resistance to cell death. Genes Dev. 2004 May 15;18(10):1131-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-392

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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