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Western blot analysis of extracts of various cell lines, using SNAIL antibody (19-550) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using SNAIL antibody (19-550) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SNAIL antibody (19-550) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SNAIL antibody (19-550) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SNAIL Antibody

CATALOG NUMBER: 19-550

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNAIL (NP_005976.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
293T
Positive Control 3:
K-562
Positive Control 4:
NIH/3T3
Positive Control 5:
Mouse lung
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Snai1
Additional Names:
SNAI1, SNA, SNAH, SNAIL, SLUGH2, SNAIL1, dJ710H13.1
Ncbi Gene Id Number:
6615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-550

List Size:
100 uL

List Price:

$436.00

Datasheet
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