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Antibody used in WB on Human Frontal Cortex at 0.2-1 ug/ml.
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate Lane 2. Mouse WT brain extract (80ug)).
Antibody used in WB on Human Frontal Cortex at 0.2-1 ug/ml.

SNAP25 Antibody

CATALOG NUMBER: 31-133

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SNAP25.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SNAP25 antibody can be used for detection of SNAP25 by ELISA at 1:312500. SNAP25 antibody can be used for detection of SNAP25 by western blot at 1.0μ μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Frontal Cortex Lysate
Predicted Molecular Weight:
23 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SNAP25 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SNAP25
Additional Names:
SNAP25, sp, Bdr, SNAP-25
Protein Accession Number:
NP_035558
Protein Gi Number:
6755588
Ncbi Gene Id Number:
20614
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Snap25 contains 2 t-SNARE coiled-coil homology domains and belongs to the SNAP-25 family. t-SNARE involved in the molecular regulation of neurotransmitter release. Snap25 may play an important role in the synaptic function of specific neuronal systems. Snap25 associates with proteins involved in vesicle docking and membrane fusion.
Background Reference 1:
Nagy, G., et al., (2005) Mol. Biol. Cell 16 (12), 5675-5685.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-133

List Size:
100 ul

List Price:

$519.00

Datasheet
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