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Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

SNAP25 Antibody

CATALOG NUMBER: 59-662

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Chicken, Mouse, Rat
Immunogen:
This SNAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the Central region of human SNAP25.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNAP25
Additional Names:
Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP
Protein Accession Number:
P60880
Protein Gi Number:
46397726
Ncbi Gene Id Number:
6616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Synaptic vesicle membrane docking and fusion is mediatedby SNAREs (soluble N-ethylmaleimide-sensitive factor attachmentprotein receptors) located on the vesicle membrane (v-SNAREs) andthe target membrane (t-SNAREs). The assembled v-SNARE/t-SNAREcomplex consists of a bundle of four helices, one of which issupplied by v-SNARE and the other three by t-SNARE. For t-SNAREs onthe plasma membrane, the protein syntaxin supplies one helix andthe protein encoded by this gene contributes the other two.Therefore, this gene product is a presynaptic plasma membraneprotein involved in the regulation of neurotransmitter release. Twoalternative transcript variants encoding different protein isoformshave been described for this gene.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Greaves, J., et al. J. Biol. Chem. 285(32):24629-24638(2010)
Background Reference 3:
Oner, O., et al.Atten Disord (2010) In press :
Background Reference 4:
Zhang, H., et al. Eur. J. Paediatr. Neurol. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-662

List Size:
400 ul

List Price:

$495.00

Datasheet
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