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Western blot analysis of extracts of various cell lines, using SNAP29 antibody (19-330) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver damage using SNAP29 antibody (19-330) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNAP29 antibody (19-330) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SNAP29 antibody (19-330) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SNAP29 antibody (19-330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

SNAP29 Antibody

CATALOG NUMBER: 19-330

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_004773.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
HeLa
Positive Control 3:
BxPC-3
Positive Control 4:
HepG2
Positive Control 5:
293T
Positive Control 6:
U-251MG
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNAP29
Additional Names:
CEDNIK, SNAP-29, synaptosomal-associated protein 29, soluble 29 kDa NSF attachment protein, synaptosomal-associated protein, 29kD, synaptosomal-associated protein, 29kDa, synaptosome associated protein 29kDa, vesicle-membrane fusion protein SNAP-29
Ncbi Gene Id Number:
9342
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-330

List Size:
100 uL

List Price:

$436.00

Datasheet
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