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43-101 (0.1ug/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-101 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-101 (0.1ug/ml) staining of Human Amygdala (A) and fetal Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SNCA Antibody

CATALOG NUMBER: 43-101

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-ATGFVKKDQLGK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Brain (Amygdala) and fetal Mouse Brain lysates (calculated MW of 14.5kDa according to Human NP_000336.1 and Mouse NP_033247.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_009292.1; NP_000336.1). Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1
Predicted Molecular Weight:
Approx 17 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SNCA
Additional Names:
SNCA, synuclein, alpha (non A4 component of amyloid precursor), NACP, PARK1, PARK4, PD1, alpha-synuclein, non A-beta component of AD amyloid, synuclein alpha-140
Protein Accession Number:
NP_009292.1,NP_000336.1
Protein Gi Number:
6806898
Ncbi Gene Id Number:
6622

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-101

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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