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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis of SNF8 using rabbit polyclonal SNF8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.
Western blot analysis in A549 cell line lysates (35ug/lane).

SNF8 Antibody

CATALOG NUMBER: 59-181

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human SNF8.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNF8
Additional Names:
Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30
Protein Accession Number:
Q96H20
Protein Gi Number:
73919323
Ncbi Gene Id Number:
11267
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) formESCRT-II (endosomal sorting complex required for transport II), acomplex involved in endocytosis of ubiquitinated membrane proteins.SNF8, VPS25, and VPS36 are also associated in a multiproteincomplex with RNA polymerase II elongation factor (ELL; MIM 600284)(Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001[PubMed 11278625]).
Background Reference 1:
Malerod, L., et al. Traffic 8(11):1617-1629(2007)
Background Reference 2:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 3:
Langelier, C., et al. J. Virol. 80(19):9465-9480(2006)
Background Reference 4:
Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-181

List Size:
400 ul

List Price:

$495.00

Datasheet
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