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Western blot analysis of extracts of various cell lines, using SNRPA antibody (22-200) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Chromatin immunoprecipitation analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (18-626) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunohistochemistry of paraffin-embedded mouse heart using SNRPA Antibody (22-200) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SNRPA antibody (22-200).
Western blot analysis of extracts of various cell lines, using SNRPA antibody (22-200) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

SNRPA Antibody

CATALOG NUMBER: 22-200

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
293T
Positive Control 3:
A-431
Positive Control 4:
MCF7
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 31kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNRPA
Additional Names:
SNRPA, U1A, Mud1, U1-A
Ncbi Gene Id Number:
6626
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-200

List Size:
100 uL

List Price:

$436.00

Datasheet
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