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Western blot analysis of extracts of various cell lines, using SNRPE antibody (19-697) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody (19-697) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using SNRPE antibody (19-697). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SNRPE antibody (19-697) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

SNRPE Antibody

CATALOG NUMBER: 19-697

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SNRPE (NP_003085.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
HepG2
Positive Control 3:
U-937
Predicted Molecular Weight:
Observed: 11kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNRPE
Additional Names:
HYPT11, SME, Sm-E, snRNP-E, small nuclear ribonucleoprotein E, sm protein E
Ncbi Gene Id Number:
6635
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-697

List Size:
100 uL

List Price:

$436.00

Datasheet
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