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Western blot analysis of SNRPN in THP-1 cell lysate with SNRPN antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of SNRPN in human spleen tissue with SNRPN antibody at 5 μg/mL.
Immunofluorescence of SNRPN in human spleen tissue with SNRPN antibody at 20 μg/mL.
Western blot analysis of SNRPN in THP-1 cell lysate with SNRPN antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

SNRPN Antibody

CATALOG NUMBER: 8155

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (95%)
Immunogen:
SNRPN antibody was raised against a 20 amino acid peptide near the amino terminus of human SNRPN.
The immunogen is located within amino acids 30 - 80 of SNRPN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SNRPN antibody can be used for detection of SNRPN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SNRPN antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 25 kDa
Purification:
SNRPN antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SNRPN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SNRPN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SNRPN
Additional Names:
Small nuclear ribonucleoprotein-associated protein N, HCERN3, PWCR, RT-LI, SM-D, SMN, SNRNP-N, SNURF-SNRPN, sm-N
Protein Accession Number:
NP_003088
Protein Gi Number:
4507135
Ncbi Gene Id Number:
6638
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The small nuclear riboprotein-associated protein N (SNRPN) is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN plays a role in pre-mRNA processing and alternative splicing (1). SNRPN arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF) (2). Mutations or deletions in the SNRPN gene may be linked to responsible for Angelman syndrome or Prader-Willi syndrome (3).
Background Reference 1:
Ozcelik T, Leff S, Robinson W, et al. Small nuclear ribonucleoprotein polypeptide N (SNRPN), an expressed gene in the Prader-Willi syndrome critical region. Nat. Genet. 1992; 2:265-9.
Background Reference 2:
Gray TA, Saitoh S, and Nicholls RD. An imprinted, mammalian bicistronic transcript encodes two independent proteins. Proc. Natl. Acad. Sci. USA 1999; 96:5616-21.
Background Reference 3:
Chen Y, Liu YJ, Pei YF, et al. Copy number variations at the Prader-Willi syndrome region on chromosome 15 and associations with obesity in whites. Obesity 2011; 19:1229-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8155

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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