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Western blot analysis of extracts of mouse spleen, using SOCS3 antibody (13-252) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using SOCS3 antibody (13-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SOCS3 antibody (13-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SOCS3 antibody (13-252) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse spleen, using SOCS3 antibody (13-252) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SOCS3 Antibody

CATALOG NUMBER: 13-252

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse spleen
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SOCS3
Additional Names:
SOCS3, CIS3, Cish3, SSI-3, SOCS-3, suppressor of cytokine signaling 3, STAT induced STAT inhibitor 3, cytokine-induced SH2 protein 3, SSI3
Ncbi Gene Id Number:
9021
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-252

List Size:
100 uL

List Price:

$436.00

Datasheet
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