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46-409 (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-409 (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SOCS3 Antibody

CATALOG NUMBER: 46-409

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PIREFLDQYDAPL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in lysates of cell line MOLT4 (calculated MW of 24.8kDa according to NP_003946.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse, Rat and Pig Liver for this particular batch.
Positive Control 1:
Cat. No. 1206 - MOLT4 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SOCS3
Additional Names:
SOCS3, CIS3, Cish3, SSI-3, SOCS-3, suppressor of cytokine signaling 3, STAT induced STAT inhibitor 3, cytokine-induced SH2 protein 3, ATOD4, MGC71791, SSI3
Protein Accession Number:
NP_003946.3
Protein Gi Number:
45439352
Ncbi Gene Id Number:
9021
Background Reference 1:
Roberts AW, Robb L, Rakar S, Hartley L, Cluse L, Nicola NA, Metcalf D, Hilton DJ, Alexander WS. Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3. Proc Natl Acad Sci USA. 2001 Jul 31;98(16):9324-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-409

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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