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46-410 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-410 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

SOCS4 Antibody

CATALOG NUMBER: 46-410

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SKVRVLRIDAPEQQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human and Rodent Brain lysates and in lysates of cell lines NIH3T3, A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans. Recommended concentration, 3-6ug/ml.
Specificity:
Both variants represent identical protein (NP_543143.1 and NP_955453.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SOCS4
Additional Names:
SOCS4, suppressor of cytokine signaling 4, DKFZp686J1568, SOCS7, SH2 domain containing SOCS box protein, suppressor of cytokine signaling 7
Protein Accession Number:
NP_543143.1,NP_955453.1
Protein Gi Number:
18254466
Ncbi Gene Id Number:
122809
Background Reference 1:
Kario E, Marmor MD, Adamsky K, Citri A, Amit I, Amariglio N, Rechavi G, YardenY. Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factorreceptor signaling. J Biol Chem. 2005 Feb 25;280(8):7038-48.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-410

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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