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Antibody used in IHC on Human kidney.
Antibody used in WB on Human cell lines at: 1:500 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in IHC on Human kidney.

SOD1 Antibody

CATALOG NUMBER: 30-113

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SOD1 antibody can be used for detection of SOD1 by ELISA at 1:312500. SOD1 antibody can be used for detection of SOD1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
16 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SOD1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SOD1
Additional Names:
SOD1, ALS, ALS1, IPOA, SOD, homodimer, hSod1, HEL-S-44
Protein Accession Number:
NP_000445
Protein Gi Number:
4507149
Ncbi Gene Id Number:
6647
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in its gene have been implicated as causes of familial amyotrophic lateral sclerosis.The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
Background Reference 1:
Hays, A.P., J. Neurol. Sci. 242 (1-2), 67-69 (2006).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-113

List Size:
100 ul

List Price:

$473.00

Datasheet
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