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Western blot analysis of extracts of various cell lines, using SOD2 antibody (14-969) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody (14-969) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SOD2 antibody (14-969). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using SOD2 antibody (14-969) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SOD2 antibody (14-969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SOD2 Antibody

CATALOG NUMBER: 14-969

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
K-562
Positive Control 2:
HepG2
Positive Control 3:
DU145
Positive Control 4:
U-251MG
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 23kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SOD2
Additional Names:
superoxide dismutase [Mn], mitochondrial, manganese-containing superoxide dismutase, indophenoloxidase B, Mn superoxide dismutase, MVCD6, MNSOD, IPOB, RP1-56L9.2, superoxide dismutase 2, SOD2
Ncbi Gene Id Number:
6648
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-969

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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