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42-386 (0.1ug/ml) staining of HeLa, HepG2, HEK293 and NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-386 (0.3ug/ml) staining of Human Cerebellum (A), Mouse Brain (B), Rat Brain (C) and Pig Brain (D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-386 (0.01ug/ml) staining of Mouse (A) and Rat (B) Spinal Cord lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-386 (0.1ug/ml) staining of HeLa, HepG2, HEK293 and NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.

SOD2 Antibody

CATALOG NUMBER: 42-386

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-EAIKRDFGSFDK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 24kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Brain and Spinal Cord lysates, while approx 26kDa band was observed in lysates of cell lines HeLa, HepG2, HEK293 and NIH3T3 (calculated MW of 24.8kDa according to Human NP_000627.2, and 24.6kDa according to Mouse NP_038699.2). Recommended concentration: 0.01-0.1ug/ml. Primary incubation was 1 hour.
Specificity:
NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1) . Reported variants represent identical protein: NP_000627.2; NP_001019637.1.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Positive Control 2:
Mouse Spinal Cord
Positive Control 3:
Rat Spinal Cord
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SOD2
Additional Names:
SOD2, superoxide dismutase 2, mitochondrial, RP1-56L9.2, IPOB, MNSOD, MVCD6, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase [Mn], mitochondrial
Protein Accession Number:
NP_000627.2,NP_001019637.1
Protein Gi Number:
67782305
Ncbi Gene Id Number:
6648

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-386

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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