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46-414 (2.5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
46-414 (2.5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

SORBS1 Antibody

CATALOG NUMBER: 46-414

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-GTFPGNYVKPLYL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:No signal obtained yet but low background observed in Human Heart and 293 lysates at up to 2ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Positive Control 2:
Cat. No. 12-341 - Human Skeletal Muscle Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SORBS1
Additional Names:
SORBS1, PONSIN, sorbin and SH3 domain containing 1, CAP, SH3D5, SORB1, SH3P12, ponsin, sh3p12, FLJ12406, KIAA1296, DKFZP586P1422, DKFZp451C066, SH3-domain protein 5 (ponsin), FLAF2, FLJ12406, R85FL, Fas-ligand associated factor 2, c-Cbl associated protein, DKFZp586P1422, OTTHUMP00000020142, OTTHUMP00000020143, OTTHUMP00000020144, OTTHUMP00000020145, ponsin
Protein Accession Number:
NP_006425.2,NP_056200.1,NP_001030126.1,NP_001030127.1,NP_001030128.1,NP_079267.1,NP_001030129.1
Protein Gi Number:
78000160
Ncbi Gene Id Number:
10580
Background Reference 1:
Ribon V, Printen JA, Hoffman NG, Kay BK, Saltiel AR. A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes. Mol Cell Biol. 1998 Feb;18(2):872-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-414

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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