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46-416 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-416 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-416 (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

SORBS1 Antibody

CATALOG NUMBER: 46-416

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-SERNDWEPPDKKVD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SORBS1
Additional Names:
SORBS1, sorbin and SH3 domain containing 1, CAP, DKFZp451C066, DKFZp586P1422, FLAF2, FLJ12406, KIAA1296, R85FL, SH3D5, SH3P12, SORB1 , Fas-ligand associated factor 2, SH3-domain protein 5, SH3-domain protein 5 (ponsin), c-Cbl associated protein, ponsin
Protein Accession Number:
NP_006425.2,NP_056200.1,NP_001030126.1,NP_001030127.1,NP_001030128.1,NP_079267.1,NP_001030129.1
Protein Gi Number:
78000160
Ncbi Gene Id Number:
10580
Background Reference 1:
Baumann CA, Ribon V, Kanzaki M, Thurmond DC, Mora S, Shigematsu S, Bickel PE, Pessin JE, Saltiel AR. CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature. 2000 Sep 14;407(6801):202-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-416

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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